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Application of the multiplex Direct PCR to detect Salmonella in pork meat at slaughter reduces the time of detection from 5 to 6 days by conventional bacterial culture and serotyping methods to 14 h (including 12 h enrichment time).
Application of the multiplex PCR for selection of shrimp with viral tolerance could be useful for future development of a program of specific resistant (SPR) shrimp.
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The sensitivity, specificity and potential applications of the multiplex assay are discussed.
Finally, the potential application of this multiplex PCR system in milk analysis is demonstrated through analysis of raw milk samples artificially contaminated with MAP.
For the application of the three multiplex PCR methods in a routine diagnostic setting, the hands-on-time, the time-to-result, the costs and the accomplishment are also very important parameters.
These advances have resulted in the appearance of numerous publications regarding the application of multiplex PCR in the diagnosis of human brucellosis.
In this article, we report on the application of a multiplex microassay platform, and we developed a rapid screening of DON and zearalenone (ZEN) in feed commodities, which is also applicable to related mycotoxins.
Here, we describe the application of a multiplex PCR (mPCR) design for simultaneous detection of four virulence genes (set1A, set1B, ial and ipaH) in Shigella spp. and to determine the prevalence of these virulence genes in a random selection of Malaysian Shigella strains.
A total of 65 Aspergillus isolates from meju were tested for the presence of aflatoxigenic fungi by the application of multiplex PCR, and were analyzed by TLC and HPLC for the aflatoxin production in the culture filtrates.
In this study, we describe the development and application of a multiplex antigen array for profiling ACPAs in RA.
To further improve the application of multiplex genome editing and to reduce the toxicity associated with multiple electroporation, we developed a One-shot CRISPR system, by incorporation of multiple gRNAs in a CAR lentiviral vector.
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