Results from an earlier evaluation of the application of site-specific 99mTc-labeling of the Affibody molecule, ZHER2 2395-C, were favorable.
The results may broaden the application of site-specific recombination in basic and applied research, including the custom-design of recombinases for natural, asymmetric, and lox-related target sequences present in the genome.
Development of 99mTc agents will likely extend the application of site-specific brain radiopharmaceuticals for routine applications in aiding the diagnosis and monitoring treatments of various neurologic and psychiatric disorders.
To this end, we envisage the application of site-specific genome-editing techniques to be game-changing.
One attractive application of site-specific nucleases is genome editing in patient-derived induced pluripotent stem (iPS) cells for the development of personalized cell therapy against intractable human diseases (Urnov et al., 2010).
ThioFabs with engineered cysteine residues in the constant domains (CL and CH1) should allow universal application for site-specific conjugation of antibody-Fabs.
Thom, J., Anderson, D., McGregor, J. & Cotton, G. Recombinant protein hydrazides: application to site-specific protein PEGylation.
Toye et al. [18] employ personal information stored on smartphones to customize the behavior of site-specific applications to best fit a particular user.
This requirement limits application of the site-specific tyrosine recombination systems in genome engineering mainly to modeling experiments.
One important application of the prenylation-based labeling strategy is the formation of site-specific protein modifications such as protein DNA conjugates, PEGylated proteins, and dually labeled proteins.
This variety of site-specific enzymes can be used to develop complex genome engineering applications at multiple loci.
