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Application of culture media from gp120-exposed BMDMs induced a significant reduction in DRG neurite outgrowth.
However, the prolonged turnaround time limits the clinical application of culture.
Another alternative would be the application of culture filtrate, since such filtrate can also promote plant growth and development (Varma et al. 1999; Ghimire et al. 2009; Vadassery et al. 2009a; Bagde et al. 2011; Kumar et al. 2012b) and influences the synthesis of particular valuable compounds (Balsi et al. 2010; Bagde et al. 2011; Kumar et al. 2012b).
Data for 1652 patients with suspected leptospirosis recruited during three observational studies and one randomized control trial that described the application of culture, MAT, immunofluorescence assay (IFA), lateral flow (LF) and/or PCR targeting the 16S rRNA gene were reevaluated using Bayesian latent class models and random-effects meta-analysis.
The inclusion and application of culture as a specific social construct in HIV intervention approaches is an innovative concept, which stemmed from the belief that by understanding cultural characteristics of a given group, public health programs would be more capable of meeting the health needs of its members [ 25].
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Community-level studies have become more precise with the application of culture-independent methods based on the direct detection of DNA in microbial ecosystems.
The application of culture-independent metagenomic approaches allows exploiting this almost unlimited resource of novel biomolecules (Sjöling and Cowan 2008).
Work in this area has been aided by the increasing application of culture-independent microbial detection tools, such as high-throughput sequencing and metagenomics.
Owing to the difficulty in accessing many members of this community, the application of culture-independent approaches to study MGE are required to further study this flexible gene pool.
One study also reported a successful application of cultures with no demonstrated bacteriocinogenic activity for extension of the shelf-life of vacuum-packed cold-smoked salmon (Leroi et al., 1996).
With the application of this culture method, fertilizable oocytes can also be obtained by culturing the follicles on flat tissue culture dishes [ 44].
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