The application of cultivation-independent methods as an alternative to assess bacterial viability is highly valuable for a number of reasons.
Reviewer 2: This is an interesting application of cultivation-independent methods for characterizing successional changes during the microbial decomposition of mice corpses.
The introduction of fingerprint-based analyses [ 26, 36, 37] turned into the application of cultivation-independent techniques as routine tools to depict the overall microbiome composition in environmental samples and to infer which factors influence the abundance and distribution of specific microbial taxa.
However, the application of novel cultivation strategies is slow and will not enable cultivation of the extant diversity of microbial genomes from extreme environments, necessitating new approaches to exploit natural products from as-yet-uncultured microbes for industrial application (Daniel 2004).
Negative effects are often induced in crop soil quality by application of intense cultivation practices (Batlle-Bayer et al. 2010) such as the excessive usage of chemical fertilizers.
Together with the application of modern cultivation technology, the rice yield has risen to about 6·0 t ha−2.
In order to obtain reliable and comparable data, the appropriate use of the RPM and application of standardized cultivation methods are of central importance.
Cultural practices to promote vigorous plant growth (i.e., watering during droughts, proper application of fertilizers, clean cultivation, fall and summer fallowing, use of heavy organic mulches or cover crops, and plowing out roots of susceptible plants after harvest) are useful for specific nematodes.
The application of greenhouse vegetable cultivation has dramatically expanded worldwide during the last several decades.
Since Zn bioavailability from some soya products is low, application of an adequate cultivation system becomes important.
It should be noted that a single application of GBH during cultivation of glyphosate-tolerant GM crops as conducted in the generation of the NK603 maize grain used in this study is not a typical farming practice.
