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One possible approach to this end is the application of assays for the detection of free hypohalous acid in solution, 16, 17 but this is an indirect method of detecting enzyme turnover and would not be applicable to halogenating enzymes that bind hypohalous acid in their active site, such as the tryptophan halogenases.
Establishing a cut off value in IHC negative tissues is required for the application of molecular assay in the place of IHC assay.
This phenomenon has negated the application of this assay as a bona fide stem/progenitor cell assay, since these cells seem to lack self-renewal activity.
Therefore the application of this assay is rather limited.
Based on the three dominant antigenic peptides, we also study the application of FP assay in detecting HBV infection.
The application of the assay in the serum sample was successfully investigated.
A project to research the application of nondestructive assay (NDA) to spent fuel assemblies is underway.
Several experimental factors of difficult control, such as variation of cell density, hinder a reliable and reproducible application of this assay.
Practical application of the assay indicated that target Vibrio species in seafood, clinical samples and foodborne outbreaks can be accurately detected.
These experiments demonstrate the application of the assay to the detailed kinetic analysis of a single inhibitor.
Details on the application of the assay for CSF have been published previously by our group [24].
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