Sentence examples for application of a secondary from inspiring English sources

Our investigation was focused on possibilities of application of a secondary exchanger for use of flue gas waste heat produced by small microturbines.

The application of a secondary discharge, inductively coupled plasma magnetron sputtering (ICP-MS) and microwave amplified magnetron sputtering, is discussed as well as the high power impulse magnetron sputtering (HIPIMS), the self-sustained sputtering (SSS) magnetron, and the hollow cathode magnetron (HCM) sputtering discharges.

VEGF, Ki67 and p53 were detected by application of a secondary biotinylated rabbit anti-mouse antibody (diluted 1 500; DAKO) followed by incubation with avidin biotin-peroxidase complex (1:200 dilution; Davidin biotin-peroxidase

We believe that this concurrence emphasizes the need for caution in applying antibody-based methods for 5-hmC detection without the application of a secondary detection method, especially when 5-hmC is expected to be a rare species.

Slides were then washed in PBS before application of a secondary Alexa 594 labeled anti-rabbit (Invitrogen) diluted 1 500 in PBS containing 1 drop of goat serum per 5 ml and incubated for 45 min at RT in the dark, then washed three times with PBS.

This was followed by application of a biotinylated secondary antibody for 24 hours at a concentration of 1∶200 (Vector Labs, Burlingame, CA).

This type of source is provided by the dye laser, in which laser emissions arise from the decay of dye molecules that have been excited into a multitude of closely spaced rovibronic (rotational-vibrational-electronic) levels by the application of an intense secondary laser signal (a process known as pumping).

The next day, sections were washed 3 times in PBS (5 min each), before application of a species specific secondary antibody (Molecular Probes) for 1 hr in 0.1%–0.3% triton/PBS.

Following the application of appropriate secondary antibodies, a standard streptavidin-biotin technique, with diaminobenzidine as chromogen, was used for visualization.

Schools that subscribe to the American Medical College Application Service will ask you to indicate your interest in an MD/PhD program and then to provide additional information as part of a secondary application.

Tissue sections received a 5 min TBS rinse before the application of biotinylate secondary antibody (1 : 200 dilution TBS) for 30 min and then a second 5 min TBS rinse.