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Most important is the initial application of a phred filter.
Application of a phred filter (q = 30) substantially mitigated, though did not wholly eliminate this skewing.
Therefore, errors show significant site and nt-specific variability regardless of the application of a phred filter for sequence quality.
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Q30: percentage of bases with a Phred value of at least 30.
Gb: Giga base; Q20: percentage of bases with a Phred value of at least 20.
These reads were of high quality with a Phred score of above +30 and a minimal length of 90 bases.
On average, 95.53 % bases of raw reads had a Q value ≥20 (the percentage of bases with a Phred value ≥20) and 91.39 % bases had a Q value ≥30 (the percentage of bases with a Phred value ≥30), with an error probability of 0.035 %.
Phred scores were calculated and reads where at least 50% of the bases had a Phred score of less than 20 were discarded [ 59].
Q20 and Q30: The percentage of bases with a Phred value > 20 and 30.
Q20: The percentage of bases with a Phred value > 20.
Phred quality scores also can be used to assess the quality of the sequence, with a Phred quality score of 20 implying a 1-in-100 1-in-100 1-in-100the base is incorrectly assigned [19], [20].
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CEO of Professional Science Editing for Scientists @ prosciediting.com