The phrase "application me" is not correct in English and does not convey a clear meaning.
It seems to be an incomplete or incorrect expression, and without additional context, it cannot be used effectively in written English.
Example: "Please send the application to me."
Alternatives: "send me the application" or "apply to me."
Briefly, 24-well plates were coated with 1% Seaplaque agarose (BioWhittaker Molecular Application, Rockland, ME) and tumor cells (2 × 105 cells in 1 ml complete medium) were plated on top of the solidified agarose.
Palumbo: An while u is about it, can ur homies sort out me tricky plannin application at me club?
Another told me that he was willing to file a green-card application for me, but that it would cost another $10,000, which we did not have at the time.
Dishes (60 mm) were coated with 1% SeaPlaque agarose (BioWhittaker Molecular Applications, Rockland, ME, USA) dissolved in DMEM/10% FBS.
The digested products were separated on a 4% NuSieve agarose gel (Bio Whittacker Molecular Applications, Rockland, ME, USA) and the genotype determined by the banding pattern observed.
Agarose was bought from BioWhittaker Biological Applications (Rockland, ME, USA) and Ready Protein+ scintillation liquid from Beckman Coulter Inc. (Fullerton, CA, USA).
A total reaction volume of 25 μl contained 12.5 μl of Master Mix, 2 μl of 25 m M MgCl2, and 0.25 μl of 25 × SyBr Green (BioWhittaker Molecular Applications, Rockland, ME, USA).
A 5% Long Ranger/6 mol/l urea gel (Biowhittaker Molecular Applications, Rockland, ME, USA) was used for rapid fragment detection using the ABI Prism 377 DNA Sequencer (Perkin-Elmer, Foster City, CA, USA) at the Dana Farber Cancer Institute Molecular Biology Core Facility and the Harvard Center for Cancer Prevention, Boston, MA, USA.
Sequencing products were purified through Sephadex G-50 columns (Sigma-Aldrich, St . Louis MO), and electrophoresed on 4.75% Long Ranger gels (BioWhittaker Molecular Applications, Rockland, ME) on an ABI Prism 377 Sequencer (Applied Biosystems).
Testing the application reminded me of this blog post published earlier this year by Loïc Le Meur about the '(de centralization of me'.
