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The following criteria can be used to determine whether β cells generated in vitro are functional and suitable for potential clinical application: expression of mature β cell markers, ability to secrete bioactive insulin and C-peptide in response to stimuli, formation of mature secretory granules and, ultimately, glucose control in transplanted recipients.
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For certain RNAi applications, expression of short hairpins offers advantages over transient transfection of siRNA.
TMAs construction and their application in expression analysis by immunohistochemistry (IHC) have been described previously [ 15].
This metric was developed for application to expression datasets consisting of unmatched experimental conditions, and it quantifies the similarity of the expression correlations between a pair of orthologs and all other identified orthologs.
Despite CHX application, Tfap2c expression level increased significantly following a one-hour GCSF application, suggesting that GCSF directly regulates Tfap2c expression (Fig. 4I).
Six hours after RaxX21-sY peptide application, the expression level of PR10b (Fig. 4a), Os10g28299 (Fig. 4b), or Os04g10010 (Fig. 4c) were assayed in Kitaake, XA21, and XA21-LRR1Ri lines.
In order to detect a possible effect of polyP on TRPM8 we conducted a number of experiments with application, or expression, of scPPX1 in HEK-293 cells.
The cross-species application of expression microarrays is equivalent to the application of poor quality microarrays.
The application of expression microarray profiling technology promises to change both our understanding of tumor biology and our clinical practices.
Another application of expression signatures is the monitoring of transcriptome changes in a community upon exposure to a toxicant [ 22].
These findings verify diagnostic utility and application of expression data for improved selection of high-risk STS patients.
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