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Our results provide proof of concept for using engineered Sortase A to produce cyclic MCoTI-II and should be generally applicable to generating other cyclic cystine-knot peptides.
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This strategy should be generally applicable to generate disulfide-bond containing proteins with functional C-terminal thioesters for subsequent native chemical ligation.
At the end, the max-error in CDF never exceeds the corresponding significant values implying that the technique proposed is applicable to generate the variables required in selection of feasible site for generation potential wind energy from turbines.
Notably, our approach and pipeline are applicable to generate LD maps of other biologically, agriculturally, and medically important mosquito species.
The methods described here for deep sequencing of the transcriptome should be widely applicable to generate catalogs of genes and genetic markers in emerging model organisms.
The approach is considered to have a promising potential being applicable to generate enniatin easily in an economic continuous fermentation process since the entire cyclodepsipeptide is secreted into the medium.
We demonstrated that the approach of animal immunization and subsequent phage display based antibody selection is applicable to generate highly specific anti β-amyloid scFvs that are capable of accurately discriminating between minute conformational differences.
While the in vivo relevance of these differences is still to be established, the study confirms that the approach of animal immunization and subsequent phage display based antibody selection is applicable to generate highly specific anti β-amyloid scFvs that are capable of accurately discriminating between minute conformational differences.
The Metropolis-Hasting algorithm is a generally applicable schema to generate the Markov chain, although faster approaches such as Gibbs sampler exist (8).
The ALKR1464STOP mutation was observed in the DNA and RNA from a patient sample analysed as part of the TARGET (therapeutically applicable research to generate effective treatments) initiative, which aims to characterise the genomic landscape of the most common childhood cancers, including neuroblastoma.
Although our experiments with real data in this work were limited to data generated on the Argus system from OpGen, similar ideas (with minor variations) should also be applicable to data generated by other technologies such as the Irys platform from BioNano Genomics.
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