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To provide a simple and robust means of aligning tomograms, we developed a universally applicable protocol for depositing colloidal gold onto vitreous sections, analogous to the method for applying quantum dots described by Masich et al. [Masich, S., Östberg, T., Norlén, L., Shupliakov, O., Daneholt, B., 2006.
Thus, this easily applicable protocol ensures that at least 99% of any kind of contaminating DNA molecules contained in the PCR reagents are degraded.
A generally applicable protocol is outlined below.
Clearly, building a predictive model based on such parameters would be a cost-effective and widely applicable protocol.
However, a clinically applicable protocol that allows the discharge of a significant proportion of patients after just a single high sensitivity troponin blood draw at presentation to the emergency department remains an attractive yet elusive goal.
3 Therefore, a clinically applicable protocol that allows the discharge of a significant proportion of patients after just a single hs-cTn blood draw at presentation remains an attractive yet elusive goal.
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Although BRT and OMT are widely recognised within osteopathy and other allied health disciplines, there are few descriptions of clinically applicable protocols in the literature.
In this regard, the implementation of bedside measurement of P-V curves and alveolar recruitment could facilitate development of clinically applicable protocols for approaching measurement of respiratory mechanics and its interpretation.
The proposed method targets time-division multiple access (TDMA) communication protocols, and is applicable to protocols such as FlexRay and TTP which have recently emerged as possible networking standards for embedded systems such as automobile controllers.
The work of our own group has been dedicated to developing a robust and clinically applicable dosimetry protocol for solid organs based on 3-D imaging [15, 21].
Here we present a novel method to efficiently retrieve the antigen in a widely applicable standard protocol, facilitating fluorescent immunostaining of both cryosections and whole mount preparations in zebrafish (Danio rerio) and medaka (Oryzias latipes).
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