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There is a growing body of evidence that metformin may be considered a promising anti-aging candidate, applicable for life span extension, prevention and even treatment of cancer [ 22- 27, 50, 56].
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Survival analyses were used for life span comparisons [47].
Mitochondrial function is very important for life span regulation [ 91].
Animals were grown as described for life span assays.
Long-lived CB1370 daf-2 (e1370) worms, a positive control for life span extension, showed the expected increase in life span in all experiments (Tab. 1a-d).
These bacteria are capable of altering life span, and can potentially result in a false positive for life span extension [ 8, 36].
For stress assays, flies were reared and housed as for life span experiments.
Plates for life span assay with DOG were prepared from the same bath of NGM agar.
Both transcription factors are required for life span extension upon down regulation of TOR [ 35, 36].
Furthermore, autophagy is essential for life span extension at least in worms [ 30].
The simulation results show that the Vickers hardness of the tested materials is approximately proportional to the heat treatment parameter P, which indicates that Larson Miller function is applicable for the life-span design of Ni-based composites.
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