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Where applicable cells were pre-incubated for 10 min with 10 μM DPI prior to stimulus addition.
Since, these cells represent a minority in most graft sources and the amount of applicable cells is limited, ex vivo expansion-cultures have been established using cytokine cocktails7,8,9 or small molecules10.
Where applicable, cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) for 15 min at 37 °C, then washed extensively.
Where applicable, cells were washed with Ca2+-free external saline (containing 10 μM EDTA to remove residual extracellular Ca2+) just before the imaging experiments.
The ability of preclinically applicable cells to facilitate remyelination in an animal model of MS would be a crucial step towards developing novel therapies.
Where applicable, cells were first incubated for 20 minutes with PD98059 (40 ng/mL), a MEK1 inhibitor from New England Biolabs (Beverly, MA) at 37°C and the adhesion assay culture medium was supplemented with PD98059 at 40 ng/mL.
Similar(52)
A reliable, clinically applicable cell-tracking agent would be a powerful tool to study cell biodistribution.
Thus, lack of applicable cell source had become a major concern that blocks clinical translation of this technology.
Systematic refinement of this two-step system using a novel clinical grade medium resulted in a therapeutically applicable cell culture protocol.
In recent years, rapid developments in regenerative medicine have identified seed cells and applicable cell stroma for tissue engineering to restore tissue damage.
Cut out each label and attach them to the toothpicks with tape before placing them on the cake next to the applicable cell component.
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