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A modified pET28b vector was designed to produce N-terminal polyhistidine-tagged proteins with a simple construction scheme having broad applicability because of the use of rare SapI cloning sites.
However, genetic manipulation has limited clinical applicability because of the predicted complications and ethical issues, and the administration of HA oligos is challenging in terms of drug delivery.
However, these approaches have not achieved real clinicopathologic applicability because of the inter-institutional variation caused by numerous factors such as; array platform, statistical algorithm, reagent, laboratory condition/protocol.
However, the acquisition of these parameters usually requires an invasive catheterization or a skilled echocardiographic evaluation to obtain an accurate interpretation of data, limiting their applicability because of the need for specialized training and equipment.
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Triglyceride micro-emulsions such as Intralipid® have been used to reverse cardiac toxicity induced by a number of drugs but reservations about their broad-spectrum applicability remain because of the poorly understood mechanism of action.
Many disease associations, especially those reported before the completion of the HGP, are limited in applicability because of potential inconsistencies with our current standards for genomic coordinates, nomenclature and gene structure.
Using only a single guide has the advantage not only of reduced technical complexity but also of increased applicability because the choice of one reactant is not limited by the required dipole moment.
Furthermore, there are still several problems in its applicability because of false-positive and false-negative reactions reported [ 15].
This test is a well- established diagnostic and prognostic tool that has widespread applicability because of its clinical accuracy and cost effectiveness [ 20].
One of the advantages of the p-values based methods for estimating <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0018874.e031.PNG" class= inline-graphic"/> is their wide applicability, because the step of calculating p-values in statistical testing is well separated from the subsequent steps.
This is because the applicability of the uniform approximation method is inherently connected to the behaviors of functions (19) and (21).
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