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Starch metabolism genes were identified and the expression of putative apple starch metabolism genes confirmed by qRT-PCR.
For example, the expression profiles of sucrose phosphatase (EB156512) and a sucrose-phosphate synthase (EB123469) mirrored the reduction in apple starch content during both early fruit development and during ripening [ 19], suggesting that these enzymes may be components of the starch degradation pathway in fruit development.
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We have identified cell division genes for which expression coincides with the period of cell division in apple fruit and have identified starch metabolic enzymes likely to be involved as fruit store and then metabolize starch.
Starch metabolism in apple fruit is a physiological process with a well-defined developmental pattern [ 19].
Matching the gene expression profiles produced in this study to known changes in starch content throughout apple development is one approach, implicating certain pathways in these processes.
Progress of starch degradation in apple fruit is commonly assessed using the starch pattern index (SPI), which is used as an indicator of when to harvest fruit for short- or long-term storage.
In total, ESTs for 15 apple genes with homology to starch metabolic enzymes were identified with microarray expression profiles that varied during fruit development (Table 4) and qRT-PCR was performed to confirm these profiles.
MIR has been applied to the detection of microbial spoilage of meat [ 18], classification of modified starches [ 19], apple juice [ 20], and the determination of fungal contamination in numerous foodstuffs.
Expression of only one of these candidate starch synthase genes in apple fruit (represented by EB121923, Figure 5) peaked at 87 DAA, just prior to the peak in fruit starch content at 100 DAA [ 19].
These are some of the apples we brought back to test starch content, firmness of the flesh, and to juice to create cider.
As an apple evaluator, I judge the russetingand bloom, the starch index at harvest.Judge is a euphemism for praise.
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