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(xi) Fluorescein was conjugated to one of the phosphine's aryl substituents through an amide linkage; a FRET quencher, disperse red-1, was appended through the ester linkage.
These modeling experiments indicate that the twist and the spiral curvature of the structure are inherent properties of a double-stranded protofibril that is uniformly assembled from the S-shaped fibrin monomers appended through asymmetric D D interactions.
For our studies we created a fluorescent labelled TTR variant with sequence C10A/V122I/P125C to which Alexa Fluor 488 dye was appended through maleimide chemistry, yielding a variant with the same aggregation and amyloid fibril formation capacity as native V122I TTR.
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This symphony sustains its "heavenly length," to cite a nickname appended to it, through an aura of hypnotic, uninterrupted beauty.
We next tested the ability of the variants to unfold a substrate with a shorter unfolded tract, 6-HIS-GFP 6-HIS-GFP 6-HIS-GFPf sin histidine residues are appended to GFP through a TEV protease cleavage site linker.
Though he saw the project through, Osiander appended an anonymous preface to the work.
Capture anti-EpCAM was then appended to the surface through NHS/EDC and biotin/avidin chemistry.
I've been a TechCrunch reader since long before I joined the masthead – and through the comments appended to each post I feel like I've got the measure of you, my fellow readers.
The second fluorophore appended to the NBD scaffold through an ether linkage provides an additional fluorescent reporter that is liberated upon nucleophilic substitution.
Although variability in the number of affected physicians across the 49 active state programmes (5 to 180 physicians) is considerable, the average programme manages about 65-75 physiciats, an an operating annual cost of about $521 000£301000000; €381 000)—paid for primarily through a charge appended to all physicians' licensing fees (about $23 for each physician in most states).
In addition we also illustrated that amino acids (other than glycine could be appended to the C-terminus 27 (through use of different amino acid loaded Wang resins) and to the N-terminus.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com