The full con promoter sequence was derived from an in vitro evolution (SELEX) protocol optimizing for binding to Eco RNAP σS-holoenzyme, but subsequent analysis indicated the sequence appeared to be optimized for binding σ-holoenzyme as well (Gaal et al., 2001).
We determined that although the atomic fluorescence detector was fundamentally linear down to at least 1 pg, the default peak integration scheme appeared to be optimized for > 10 15 pg cycle−1 and so could introduce non-linearity in smaller peaks (i.e. lower mass loadings).
Copper retention, in contrast, appears to be optimized at an intermediate salinity consistent with brackish water, and therefore may release considerable fractions of retained copper at higher (e.g. seawater) salinity levels.
Copper retention, in contrast, appears to be optimized at an intermediate salinity consistent with brackish water, and therefore may release considerable fractions of retained copper at higher (e.g. seawater) salinity levels; copper retention also appears to become more variable and dependent on aggregation mechanism at these increasing salinities.
The genetic code appears to be optimized in its robustness to missense errors and frameshift errors.
Therefore, the fast kinetics of ChRGR appears to be optimized at pH 7.4.
The intrinsic dynamics of proteins appears to be optimized by evolution for functional interactions, yet the ligand selects the one that best fits its structural and dynamic properties among the conformations accessible to the unbound protein.
In this study, using a decontamination protocol that clearly was effective for BACTEC (93.8% recovery rate of positive specimens, 7% contamination rate) does not appear to be optimized for the MGIT system (81.9% recovery rate, 18% contamination rate).
Self-regulation, including emotion regulation, appears to be optimized by a parent child relationship that has a mutually responsive orientation, meaning that interactions between parent and child are cooperative, coordinated, and harmonious, and involve the display of positive emotion [ 16, 40, 41].
It should be noted that although the enzyme's binding site does not appear to be fully optimized for a particular substrate's acyl chain, the presence of this acyl substituent does appear to be required to prevent the nonproductive binding orientation observed with l-homoserine (12), which lacks this acyl substitution.
