The orientation among independent eutectic colonies is random owing to the random appearance of nuclei throughout the volume of undercooled melts.
H&E staining of gastric TLOs further confirmed the presence of plasma cells with the characteristic appearance of nuclei and abundant cytoplasm in the circumference of the TLOs (Figure S1B, see arrows).
Histological evaluation of skin samples from these regions revealed a significantly thickened epidermis with an increased number of spinous/suprabasal cell layers and cellular disorganization; the upper differentiation layers were also abnormal, with parakeratosis, the prevalent appearance of nuclei, an improperly packed granular layer and a thicker stratum corneum (Figure 7B).
Another notable aspect was the elongated appearance of nuclei in a fraction of cells.
Nucleation of HAP begins with the formation of amorphous calcium phosphate precursors (ACP), and a relatively long induction period precedes the appearance of nuclei.
Electron microscopy analysis confirmed the effect of MAPK/JNK inhibition on nuclear morphology, even in the cortical lens fiber zone where the appearance of nuclei was suggestive of early stages of nuclear fragmentation (Fig. S8).
The proliferative zone starts at the distal tip of the gonad arm and continues until the appearance of nuclei with crescent-shape DNA morphology, characteristic of leptotene/zygotene (transition zone) germ cells as defined previously (Crittenden et al. 2006).
Dehydration issues appeared to be minimal, based on the insignificant cytosolic extraction, and freezing artifacts were also, in general, absent as indicated by the intact appearance of nuclei, the delineation and structural integrity of the nuclear, vesicular, and plasma membranes, and preservation of myelin sheaths.
Our inferences of m j + and m j − for the two nucleic markers are constrained such that budding precedes the appearance of nuclei at the bud neck (bud+< nucneck+) and nuclei only divide after appearing at the bud neck (nucneck+< nucdiv+).
As previously [7], we defined the RTN/pFRG area as located below the caudal part of the facial motor nucleus (n7), rostral to the appearance of the nucleus ambiguous.
