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As shown in Fig. 5C, IGF-2 promoted partial differentiation of PRLR KO cells, with appearance of foci of mature adipocytes.
The time course of IR repair in vivo has been assayed by observing the post-irradiation appearance of foci of the various HR repair proteins tagged with green fluorescent protein [1].
In particular, host survival is phagocyte dependent, a large inoculum is required, and infection results in the appearance of foci akin to abscesses (Prajsnar et al., 2008).
A closer inspection using confocal laser microscopy revealed a smaller and a more fuzzy appearance of foci in HCC1395 cells compared with MCF10A.
The appearance of foci requires the catalytic glutamate of ABOBEC3A, strongly implying that DNA cytosine deamination leads to DSBs in human cells.
The appearance of foci of cells resistant to terminal differentiation in untreated SENCAR cultures supports the evidence from in vivo studies for the existence of a constitutively initiated cell population in SENCAR mouse skin.
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However, the authors failed to indicate that, in the study they cited [ 16], there was no quantification of the frequency of such foci and that the appearance of these foci in the breasts of non-lactating women was significantly associated with the use of contraceptive hormones available in the late 1970s.
Without 17AAG treatment, Rad51 foci show up quickly after carbon ion irradiation (top panel in Fig. 6A), while the appearance of Rad51 foci was significantly delayed in 17AAG pretreated samples before carbon irradiation.
Fluorescence microscopy was used to observe the appearance of viral foci, indicative of recovered, infectious recombinant RABV.
The appearance of DNA foci in different transformations coincided precisely with SA-ß-gal activity and the onset of senescence.
Later, in telophase, this nondisjunction is apparently resolved via putative chromosomal breaks, which were identified by appearance of the foci of phosphorylated histone H2AX (Fig. S2).
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