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To further confirm that the impaired reprogramming efficiency was not due to the mdivi-1-induced inhibition of established iPSC colonies, we treated MEFs with mdivi-1 either at early stages (days one to seven post-viral transduction) or at later stages of reprogramming (days seven to 14 post-viral transduction) but before colony appearance (day 10) (Fig. 2B).
According to the subjects' evaluation, the highest degree of improvement occurred at distinct moments for different outcomes: day 90 (hydration and overall appearance), day 150 (smoothness), and day 210 (wrinkles, fine lines, solar melanosis, erythema, radiance, and sebum) (Table 2).
Notably, the initial beating cells were observed unexpectedly in an attempt to repeat the generation of CiPSCs, although the appearance of CiCMs were much earlier (one week after induction) than the reported earliest date of CiPSC appearance (day 20).
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