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According to morphological appearance, cell death can be divided into apoptotic and necrotic death (Kroemer et al., 2009).
Even in very old worms that were close to death as assessed by age, movement and appearance, cell nuclei were visible as distinct spots of GFP.
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Other nodal regions showed long, ca 200-500 μm, epidermal-like cells in which their jagged-in-appearance cell walls were heavily silicified.
The nucleus of these cells seem to have increased evidence of nuclear membrane breakdown with chromatin disorganization and severely shrunken appearance of cell death (Fig. 14c).
Furthermore, the FS/SS graph shows the appearance of cell fragments.
UV-B caused the gradual appearance of cell death irrespective of light conditions.
Briefly, each frame of a stack was flattened to remove regions of uneven illumination and enhance appearance of cell boundaries.
Moreover, T. brucei AOX overexpression reduces ROS generation and consequently prevents the appearance of cell death phenotypes [ 68, 87].
The observed metabolic changes are correlated with the induction and appearance of cell cycle arrest and apoptosis-related molecular markers.
All tested K9TCC cells had polygonal morphology, except K9TCC 4Molly cells that showed more flatten appearance of cells.
Figures 1A C show the appearance of cells co-cultured for 5 days and Figure 1D the appearance of cells co-cultured for 7 days.
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