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Frith, et al., found that about one-tenth of mouse cDNAs have apparent frame disruptions [ 16].
Some dmRNAs have apparent frame disruptions from 5' and 3' insertions of retrotransposons (24/325, 7%), or from an overlapping antisense gene (45/325, 14%).
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We also found some ORFs with apparent frame-shift or truncation mutations involving single neucleotide insertion or deletion.
To further understand these apparent frame-shifts, we sequenced a stock of C. autoethanogenum DSM10061, purchased directly from the DSMZ culture collection, using Illumina MiSeq technology and mapped these reads onto the Brown et al. finished genome sequence.
The 454 sequencing technology is prone to producing apparent frame-shift errors via erroneous insertions or deletions of extra bases [ 30], limiting the quality and length of codon alignments and blastX hits.
As a quality control step, we analysed the apparent reading frame of each uORF with respect to that of the true ORF, to test whether there was any frame bias (see Additional File 3).
But it's worth it, with its bittersweet flavour apparent from frame one: self-serving frontman Larry, haplessly ambitious sidekick Hank, hard-assed producer Artie.
The single synonymous mutation in the apparent reading frame is at position 384, which lies within the homeobox region.
Of the three mutations in NANOGP1's apparent reading frame, two are nonsynonymous and one is synonymous.
In control samples, Myo8A-GFP linear trajectories are apparent in a frame average of 25 frames from approximately 2 s of real time, but absent in cells treated with LatB.
Baron Cohen's uptight bourgeois sexual neuroses are apparent in every frame.
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