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Apparent calibration in the large (overall) for the marginal risk calculation was almost optimal.
The standard model showed excellent apparent calibration in the large, when estimated with the overall performance estimates (Table 2).
The apparent calibration intercept and slope were ideal for standard model when assessing the overall performance, and ideal for the marginal risk calculation when assessing the within cluster performance.
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For instance, the difference in apparent and test calibration slopes (overall) was 0.04 for both models (Table 3).
This approach led to the determination of the apparent recoveries with external calibration.
For the calculation of the extraction recovery (RE), the obtained mean values for the apparent recovery using internal calibration (RA int).
However, when matrix-matched calibration or apparent recovery for the correction of results is used, it still needs to be considered that urine samples can differ in their concentration, thereby influencing matrix effects.
However, for flow simulation, the land use input conditions had no apparent effect on the model calibration and validation results.
The fact that discrepancy in gender effect was causing unsatisfactory recalibration performance was more apparent when we evaluated the calibration quality separately for females and males (Table 6).
Nematode CBS-1 exhibited a tailing peak with a retention time of 5.776 min; on the basis of the calibration curve, the apparent native molecular mass of the protein was determined to be ~170 kDa.
In the case of external calibration, the obtained apparent recoveries for aflatoxins and DON ranged just between 35% and 50%, whereas enhanced values between 127% for T-2 and 356% for FB1 were observed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com