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We tested individual samples using the Surfblot apparatus (Idea Scientific, Minneapolis, MN, USA) which allows one to test individual serum samples without cutting the nitrocellulose membrane.
Following gel electrophoresis, RNA was transferred to a positively charged nylon membrane (GE Healthcare) in BPTE using the Genie apparatus (Idea Scientific) at 12 V for 1 h at 4 °C.
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For sera immunoblots, membranes were placed in a Surf-Blot apparatus (#5055, Idea Scientific, Minneapolis, MN) and probed with sera diluted at 1 200 in 5% non-fat milk in TBST for 2 hr at room temperature.
Out this morning in the New York Times is a stark tale: The United States' intelligence apparatus has little idea what Edward Snowden took, despite spending half a trying to find out.
Watching the clip it's as if UNC's entire basketball apparatus have no idea that selling out to stop this layup at the expense of leaving an three point shooter open is quite literally the worst thing they could do.
Chris took this new knowledge in, past the usual apparatus of his ideas and ironies, crowded as a junk yard.
Even though Turkey's president Erdogan has a vise-like grip on power, he and his intelligence apparatus apparently had no idea it was coming.
Their spectacular trophic radiations have been widely assumed to be due to structural key innovation in pharyngeal jaw apparatus (PJA), but this idea has never been tested based on a reliable phylogeny.
His firm, Nuytco Research, boasts a client list that includes the BBC, Greenpeace and film director James Cameron – Nuytten provided ideas and apparatus for The Abyss and Titanic and the pair remain friends.
Kenneth M. Young, of Skidmore, Owings & Merrill, architects, had studied the situation for six months before he got the idea for the apparatus, which Otis Elevator Co. helped him design and is now building.
I've no idea what this apparatus costs but if you're one of those "determined" types, you might be able to build something like this yourself.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com