Sentence examples for apparatus applied from inspiring English sources

One result was that the critical apparatus applied to his work was not appropriate.

Thermal conductivity is a key parameter when designing apparatus applied in related industries.

The apparatus applied an equiaxial cyclic stretch of 20% elongation to the wells at a frequency of 60 cycles/min (1 Hz).

The PCR was conducted on a 7000 Real Time PCR Apparatus (Applied Biosystems, Foster City, CA, USA), and conditions were 42 cycles at 95°C for 5 s and 60°C for 31 s.

Selected CNVs detected in the patient group were validated by real-time quantitative PCR (qPCR) [ 29] using the SYBR Green system (Roche Applied Science, Indianapolis, IN, USA) on a 7500 Fast Real-Time PCR System apparatus (Applied Biosystems, Foster City, CA, USA).

Expression of TNF-α was analyzed in triplicate on ABI7900 apparatus (Applied Biosystems) and normalized to hypoxanthine-guanine phosphoribosyltransferase (HPRT) housekeeping gene.

For micro-RNA, reverse transcription was performed using Universal cDNA Synthesis Kit II (Exiqon), and miR-146a expression was determined by real-time qPCR using ExiLENT SYBR® Green (Exiqon) on ABI7900 apparatus (Applied Biosystems).

After having assessed 5 µl of each purified PCR product on a 2% (w/v) agarose gel stained with ethidium bromide, corresponding amplicons were sequenced in both directions on an automated ABI 3130 Genetic Analyser apparatus (Applied Biosystems, Nieuwekerk, The Netherlands), using the BigDye Terminator v3.1 Cycle Sequencing kit from the same manufacturer.

Fragments were separated on an ABI 3100 apparatus (Applied Biosystems, Foster City, CA).

qRT-PCR was performed in duplicate using the Power SYBRGreen PCR Master Mix (Applied Biosystems) in an Sequence Detection System 7900HT apparatus (Applied Biosystems).

The following thermal profile was used on an ABI Prism 7000 SDS Real-Time apparatus (Applied Biosystems): 30 min at 45°C for reverse transcription; 2 min at 95°C to inactivate the reverse transcriptase and activate the DNA polymerase; and then 40 amplification cycles of 15 s at 95°C and 1 min at 60°C each.