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Cell apoptosis was estimated using DNA fragmentation ELISA.
Apoptosis was estimated using hoechst pentahydrate (bis-benzimide) stain (Invitrogen, ON, Canada).
Neuronal apoptosis was estimated using the Annexin-V Fluorescein (FITC) apoptosis detection kit (Oncogene Research Products, San Diego, CA, USA) according to the manufacturer's instructions.
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RNA concentration was estimated using nanodrop spectrophotometer.
Protein concentration was estimated using Coomassie Plus Protein Reagent.
Nucleotide diversity was estimated using DnaSp 4.10 [38].
Statistical power was estimated using 1000 simulations.
Protein concentration was estimated using the Micro BCA Protein Assay Kit Thermoo).
Confidence intervals were estimated using normal approximation.
Divergence times were estimated using Multidivtime [52] [54].
Effects were estimated using conditional logistic regression.
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