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Analytical profile index (API) strip tests and 16S rRNA gene sequencing were carried out for the identification of the genus to which the strain belong.
If these tests still proved equivocal, further species identification was carried out using the API strip identification system (API 20 and API 20 NE; bioMérieux UK Ltd ,Basingstoke, UK).
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More recently, isolates collected from the intestines and gills of Pangasius fish were identified by API strips as Enterobacteriaceae (49.1 % of the isolates), pseudomonads (35.2 %) and Vibrionaceae (15.7 %) (Sarter et al. 2007).
Blood culture isolates were identified using standard microbiological procedure and further confirmation was done using commercially available API strips [ 12].
The strength of the colour change in the API strips was read visually and judged on a scale 0 5 as suggested by the manufacturer.
The BioMérieux API CAMPY strip consisted of 20 microtubes containing dehydrated substances, with each microtubule corresponding to an individual test.
The colonies were typed by API Staph strip (bio-Mérieux) and tube coagulase test was performed (Canicatti and Roch [1989]).
For the API 20NE strip inoculation, test nitrate reduction (NO3) and p-nitrophenyl-β-D-galactopyranoside hydrolysis (PNPG) were inoculated by distributing the saline suspension into the tubes using Pasteur pipette.
Identification was confirmed by API 20E strip test (bioMérieux, France).
FRC24 was identified by using the API Coryne strip (bioMérieux).
The microorganisms were identified as S. sonnei by the API 20E strip (bioMerieux Italia, Florence, Italy).
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