Apart from the PRC2 binding at its 5′-end, indeed, HOTAIR binds through its 3′-end the lysine-specific demethylase 1 (LSD1), the enzyme that, complexed with the corepressors REST (RE1-silencing transcription factor) and CoREST (corepressor for element 1-silencing transcription factor), erases the activating H3K4 trimethylation.
Apart from the direct binding, FoxO6 can also repress PGC-1α transcription either by blocking the activity of other DNA-binding transcription factors through protein protein interaction or by regulating the expression of other upstream transcription factors.
Apart from the DNA binding region, Bcl11b possesses domains responsible for transcriptional regulation.
Apart from the NAD-binding domain with a core Rossmann-type fold found at the N-terminal region of all oxidoreductases, no significant similarity at the sequence level was observed between DXR-I and DXR-II to infer homology [ 23].
Apart from the protein-binding motifs (LIG), the ELM database also contains motifs forming proteolytic cleavage sites (CLV), post-translational modification (PTM) sites (MOD) and sub-cellular targeting sites (TRG).
Interestingly, apart from the PI-binding site, the second region possessing highly positive charges expands from the Ex to CC subdomains (the region with yellow broken lines in Figure 7A) and extends to the side wall of the domain towards the bottom side.
While it was suggested that PAD4 recognizes five successive residues with the consensus sequence of ΦXRXX, where Φ denotes amino acids with small side chain moieties and X denotes any amino acid, it is evident from both the lack of strong sequence specificity and the available PAD4 crystal structures that there are no obvious substrate-specificity determinants apart from the arginine-binding site.
Put the hay apart from the bedding.
Also, the magnitudes of the impacts of the MMs and base bulges on the hybridization signal are very similar (apart from the relative high binding affinity of Group II bulges).
Interestingly, this binding site is located only ∼20 bases apart from the reported Runx-binding site.
