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The porosity of the A + P structure was greater compared to that of the AP structure.
We conclude that the partial opening of the AP structure during the H-D exchange of tryptophan 109, although very slow, is not a rate determining step in the unfolding of this protein.
Alkaline phosphatase (AP) structure and active site.
Similar(57)
The core fold and the active-site architecture of the H. salinarum enzyme are similar to other AP structures.
We have included both parallel (P) and antiparallel (AP) structures for comparison.
Sequences and secondary structure information obtained from the AP structures were mapped onto a preliminary MSA and sequences that appeared to lack important β sheets were removed.
Sequence LOGOS were created with WebLogo (Crooks et al. 2004) (http://weblogo.berkeley.edu/, last accessed June 13 , 2014, and AP structures were visualized and edited with Jmol (www.jmol.org, last accessed June 13 , 2014.
At present it is not clear whether this second minor Man8GlcNAc2-AP component and the Man7GlcNAc2-AP structure represent slow processing of Glc1-0Man9-8GlcNAc2 oligosacytosolic by cytosolic mannosidase or whether they arise directly from either LLO or glycoprotein.
The AP-2 α–σ2:Nef structure was first aligned with the open conformation of AP-2 core structure (2XA7), and then docked on the membrane.
The AP-aldolase structure reveals the molecular basis of a hereditary disease and represents one of only a few structures known for mutant proteins at the root of the thousands of other inherited disorders.
Mildly acidic-pH alone did not cause any disruption in AP-HDL structure as judged by gel filtration (not shown) and in agreement with recent reports that HDL is a highly stable structure [38], [39].
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