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Volume-conducted ECGs were recorded simultaneously with the AP recordings.
Action potential (AP) recordings suggested that most iPS-CMs measured up to day 23 of differentiation are of ventricular-like type.
Action potential (AP) recordings from single cells were done using the whole-cell patch-clamp technique.
Myocytes were isolated as previously described [40] using standard intra- and extracellular solutions for AP recordings (see [40] for details).
To understand the electrophysiological properties of our hESC-CMs, we took action potential (AP) recordings from single cells using whole-cell patch-clamps.
Different definitions of the QT interval, as depicted by the cursors, were compared with predictions from AP recordings.
Similar(45)
Despite that match, the pulse structure of the AP recording did not match any of the field recordings in Walker's database.
For computed averages of mIPSCs, sIPSCs, and AP-IPSCs, the fast rising phase of the IPSC, the fast rising phase of the presynaptic AP (dual recordings) or the extracellular stimulus was used, respectively, as a trigger.
In the present study, this result compliments our MAP recordings from isolated perfused hearts, in which we demonstrated that APs recorded from the endocardial surface were greater in duration than APs recorded from the epicardial surface.
The model AP resembles APs recorded from human atrial samples and responds to rate changes, L-type Ca2+ current blockade, Na+/Ca2+ exchanger inhibition, and variations in transient outward current amplitude in a fashion similar to experimental recordings.
CA2 dendrites had distinct electrophysiological properties from CA1 dendrites, including lack of voltage sag, a late depolarizing response to hyperpolarizing current injection characteristic of Ih, and lack of AP adaptation (CA2 recordings of Figure 8B compared to CA1 recordings in Figure 8 figure supplement 1).
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