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The AOC protein provides a precursor for JA biosynthesis [ 47].
Accumulation of AOC protein in stem bases of cuttings of Petunia hybrida expressing PhAOC -RNAi.
This reduced level of PhAOC-transcript caused a reduction of AOC protein contents to undetectable amounts as shown by immunoblot analyses (Additional file 1: Figure S2).
At specific time points, 1 cm of the cutting base (rooting zone) was used for counting of root primordia and roots, immunological detection of AOC protein or was immediately frozen in liquid nitrogen and stored until use at −80 °C.
This is in agreement with data showing that not only JA perception but also JA formation is required for female development: AOC protein occurs specifically in ovules, and OPDA, JA, and JA-Ile accumulate preferentially in the ovary of flower buds, where the levels markedly exceed those detected in non-stressed leaves [ 24].
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For all of the19 queried sequences, the same three top-scoring proteins were found, all of which belong to the allene oxide cyclase-like protein (AOC) family.
AOC barrel-like protein d2brja1, which shared only 17 26% sequence identity among the IiDIRs, was predicted as a DIR homolog with about 98% probability, followed by two hypothetical proteins with similar probabilities (d1zvca1 and c4h69A, Table 2).
T-AOC was calculated using OD as T-AOC level (U/mg protein) = (OD sample − OD blank)/0.01/30 × dilution of reaction system/sample protein concentration.
Patients with antioxidant vitamin intake below 66% of RDA had PL, CO and AOC levels of 2.4 nm/mg protein, 2.6 nm/mg protein and 1.5 nm/mg protein, respectively, at ICU admission; at day seven of the ICU stay these values were 2.9 nm/mg protein, 3.5 nm/mg protein and 1.1 nm/mg protein, respectively, and the change was significant in all cases (P < 0.01).
Finally, differences in immunohistochemical profiles between AOC and non-AOC were also noted (Table 1).
Veterans also experienced episodes of AOC without LOC (only AOC).
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