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It is based on the constant-pressure variable-volume method and is able to work with any traconstant-pressure variable-volume
This suggests that the method can be used with any tracer, as long as it is injected as a rapid bolus in a standardized fashion.
In contrast, our autoradiography results of rat brain did not show any tracer uptake in the investigated GLP1-R-rich areas after injections of either high (50 MBq) or low (5 MBq) doses of radioactivity.
The uptake of any tracer depends on tracer delivery (perfusion) and actual uptake in cells (transporters).
A key advantage of this approach is that it allows us to measure at the bed-side and without any need to utilize any tracer.
For these assays, the perfusion medium contained 10 μmol/l cytochalasin B, 10 mmol/l glucose (to compete for re-uptake of any tracer that leaked out of cells) and an excess level of pyruvate (10 mmol/l) was included as oxidative fuel to compensate for glucose transport blockade during dye-transfer assays.
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Whether the test-retest metric of any given tracer is adequate for any particular clinical study depends on the effect size being investigated.
Thus, KVM is instrumented with tracepoints which can be traced using any kernel tracer.
One of the critical characteristics of any conservative tracer is its stability under various reservoir conditions.
By this method, brain networks can be investigated in (denoised) dynamic data obtained with any PET tracer.
This allows modelling any desired tracer distribution while providing reference data or ground truth useful for a number of quality assurance purposes.
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