Criteria for selection of a replacement method included ease of use, analysis time, cost, safety, sensitivity, accuracy, and precision.
This method is often referred to as the phantom replacement method or the replace and match method.
The epitope-tagged genes were used to replace their endogenous versions by two step-gene replacement method [36].
Unlike the insertion method, lipid molecules wrap around the protein such that the replacement method can be used for virtually any kinds of membrane proteins, including transmembrane proteins, monotopic proteins, and interfacial peptides.
On the other hand, the replacement method can generate a lipid bilayer for virtually any kinds of shape and size of membrane proteins.
The replacement method uses a packing of lipid-like pseudo atoms, and replaces them by lipid molecules one at a time.
For those and other reasons, "urologists agree that a need exists for a new replacement method," said Dr. Steve Y. Chung of the Advanced Urology Institute of Illinois.
The phases were determined by the molecular replacement method with the atomic coordinates of the unliganded TM0415 (PDB ID 1VK4) using the program Molrep39.
The structures were solved by the molecular replacement method with the Phaser program using the structure of the human H3.3 nucleosome (PDB ID: 3AV2) as the search model64,67.
The structure was solved by the molecular replacement method implemented in Phenix Phaser-MR43, using the model of C1C2 (PDB: 3UG9).
Galvanic replacement method is still the most used and prospective method in this field.
