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We also assayed Smed-slit mRNA expression by WISH in Smed-ima-1 knockdown animals and did not find any overt difference in its pattern or levels (Fig. 5f).
We did not find any overt difference in cycling cells or cytoarchitecture in the subependymal layer in regions containing an intact ependyma between Subject 1 and Subject 2. However, this was based on a snapshot view at the end of life.
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Although this may be considered a limitation of the present study, our own previous results by using osteoblasts isolated from bone tissue underlying lesional and non-lesional areas of cartilage did not show any overt differences in terms of phenotype or behavior [ 71].
Besides, we could not detect an overt difference in the PrPSc deposition as determined by immunoblot (Fig. 7C).
Sections from the left (siRNA-infused) and right (untreated) hemispheres, which were processed in parallel, showed an overt difference in mRNA labeling, with less robust signal intensity and decreased number of labeled neurons in the left substantia nigra of all 3 animals receiving α-synuclein siRNA (Fig. 2A).
Ultrastructural analysis of neuronal cells inside the VTA did not reveal any overt difference between WT and PGC1α-KO mice, neither in mitochondrial density, nor in the intracellular distribution of mitochondria (Additional file 2: Figure S2).
If there is no overt difference in behavior in any set of circumstances between a system that understands and one that does not, evolution cannot select for genuine understanding.
Our results indicate that there is no overt difference in PR expression as a function of BRCA mutation status.
cadps2 (cpd2) is broadly expressed in both the left and right dHb [ 16], and expression was similarly robust and symmetric in tcf7l2 mutants at 4 dpf, suggesting that there is no overt difference in the size of the dHb.
Scanning electron microscopy (SEM) on limb buds from E11.5 embryos revealed the presence of cilia on both wild-type and hitchhiker mutant cells, with no overt difference in their length or morphology (Fig. 11D I).
Histological analysis (Hematoxylin and Eosin staining) evaluating pathologic changes including edema, cell vacuolation and necrosis did not reveal any overt differences between cerulein-treated and untreated mice in this acute timeframe between treatment and euthanasia (data not shown).
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