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Any marker that was not found in all species was excluded.
Nevertheless, to date, we do not have any marker that could help to predict BM evolution.
Any marker that was not a causal variant but was deemed significant by both MLMM and CMLM was considered a false positive (Table 1).
Genotypes were set to exclude any marker that was genotyped in <160 individuals (80% of total number of haploids) and provide generic output.
Markers with minor allele frequency <1%, SNPs with >5% missing genotypes and, any marker that failed an exact test of Hardy Weinberg equilibrium (P < 10−7) were excluded from further analyses.
As expected, Rho1 serves as an effective positive control for image-based assays, as treatment with Rho1 dsRNA results in a binuclear phenotype readily detectable using a DNA dye and any marker that defines the cell body.
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Any markers that do not map to genes or unannotated genes are eliminated from this analysis.
Nevertheless, we recommend ongoing monitoring of DArT-based results to flag any markers that demonstrate symptoms of instability.
Any markers that showed inconsistent physical position in the RefGen_v2 with themselves were excluded from further mapping.
Any markers that remained 'unlinked' following co-segregation analysis or that were contained in groups of fewer than six markers were visually inspected in (Illumina).
Previous work has identified that it is worth retesting any markers that fail to amplify at the first PCR attempt [ 21].
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