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It is also shown that any of the following modifications allows the construction to work: (1) coloring the edges of the tiling and imposing rules on which colors can touch; (2) allowing the tile to be multiply connected; (3) requiring maximum density rather than space-filling; (4) allowing the tile to have a thickness in the third dimension.
However a cleavable sequence may be exposed due to any of the following modifications: (a) exosite binding [37], [43], (b) post-translational changes, (c) binding of allosteric effectors, (d) unfolding by chaperones and (e) cleavage of a well accessible alternate site by the same or a different protease.
The published in situ hybridization protocol [ 53] was followed with the following modifications.
Our invariant reconstruction method follows [ 11] with the following modifications.
The TD basically followed the AFLP method described above with the following modifications.
Analytical methods followed those described by McCulloch et al. [21] with the following modifications.
The following modifications made a difference for study participants.
Dissociated neuron culture was performed as previously described45,64 with the following modifications.
That said, the following modifications have been made to prevent viral replication.
RNA from salt bladders was isolated using the NucleoSpin RNA Plant Kit (Macherey-Nagel) according to the manual with the following modifications.
Single amplified genomes (SAGs) were generated and their taxonomic assignments were obtained as in Martinez-Garcia et al.40 with the following modifications.
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