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Additionally, no proteins that cluster within this clade were identified in sorghum, maize or any EST library.
PDE4B truncated super-short forms were not detectable in any EST libraries.
UniGene Present or Not Found denote whether the gene had any EST evidence or not, respectively.
PDE4C short and super-short transcripts were not detectable in any EST libraries screened.
A challenge for any EST project is obtaining sufficient coverage of less abundant transcripts [ 24].
For the BfloGATA456 ortholog, we were unable to identify any EST from the pre-release database.
Similar(29)
ESTs were filtered for length a second time and any ESTs less than 100 bases or with low complexity sequence greater than the threshold of 50% were removed.
We also determined whether any EST-SSRs matched previously published passerine microsatellites.
We did not identify any ESTs encoding BnaABF2.
To date, blast searches of the EST databases has not identified any ESTs for human ACE3.
Finally, we could not find any ESTs relevant to PNPLA1 in the chicken EST-databases [ 27, 28].
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