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In the responding patients (as judged by disease in non-osseous sites), any detectable response on bone scan is often delayed by up to 6 8 months and it can take over 2 years for complete resolution of bone scintigraphy findings (Scher, 2003), even when all of the disease has been eliminated from the bone.
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All other peptides had all their responses included i.e. any peptide that had a detectable response on at least one slide had its responses from all slides analyzed, whether or not these were above or below the cut-off.
Since no peptides are expected to give a detectable response on a slide with only buffer and secondary antibody, the responding peptides on the buffer slides were considered as false positives.
The lowest dose of NVP-AUY922 which could elicit a detectable response on HSP90-p23 complexes as well as AKT phosphorylation was determined.
Every spot with at least one detectable response on one slide was retained in the analysis.
In Figure 1A, it did not show any detectable response in the absence of TCh.
Octopamine and pilocarpine did not produce any detectable response up to 10 µM.
If a peptide had no detectable response on any slide it was excluded from further analysis.
We also excluded any spot that had no detectable response on all slides.
In our hands, the 16-hour detection platform of the ELISpot is more sensitive for IFNγ detection than the 6-hour detection platform of the flow assay, which may account for the lack of detectable responses on intracellular cytokine staining.
They all showed severe RCD with constricted visual fields, no detectable responses on full field electroretinogram, and both peripheral involvement and macular involvement in family F752 as an example).
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