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While the SDM is abstract in nature, it defines a concrete framework that can be easily implemented in any database and does not constrain the data and metadata that can be stored.
It should be noted that there are many decades of ground-based measurements that have not yet been compiled into any database, and that form our only record of pre-satellite era deformation (e.g., deformation during unrest at 27 volcanoes described by Phillipson et al. (2013) and extracted from Global Volcanism Program weekly reports).
The other 5,269 NITs (60%) had no match in any database and appear to be unique to An. gambiae (Additional file 2: Table S2).
Furthermore, we excluded from our gold standard database personal communications, because they cannot be retrieved by any database, and abstracts because it has been clearly demonstrated that such abstracts often display non-valid results [ 21, 22].
Manual annotations gave a total of 317 probe sets (61%) similar to a known protein, 80 (16%) identified genes and 121 (23%) lacking any sequence similarity in any database and therefore called "unknown" (for a detailed list see Additional file 1).
"tRF5-GlyGCC (A to C)" has been assigned as a tRF-5, because we failed to find its identical sequence in any database and it mapped to tRF5-GlyGCC perfectly, except that the third nucleotide was C while the correct one in the mouse genome database is A. As seen in Figure 1A-B, there were several tRF isoforms that were of an identical 5'-end but of different 3'-ends.
Similar(54)
The remaining 3% yielded no hits with any databases and were considered unclassified (Fig. 2).
I have an easy-to-use Filemaker template that I use for just such a database, and any information that I pick up in my daily networking goes directly into that file so that I can access it later.
The results were entered into a database and any discrepancies between the reviewers were subsequently resolved by consensus.
Data were manually entered into a database and any outlying responses were checked against the original questionnaires.
Twenty-two oft of 29 were previously not described in any database (novel), and six of these 22 were nonsynonymous.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com