Carter returned to Tokyo, set up house with Araki, and soon found that she had to acquire an additional sort of freedom.
Following an additional sort to obtain populations of DCs that had captured or not infected or uninfected neutrophils, the DCs were cultured with naive CFDA-labeled OT-I CD8+ T cells in presence of soluble OVA.
Following culture the epidermal cells were first enriched by AutoMACS with anti-MHCII-PE and anti-PE microbeads (Miltenyi Biotec), followed by an additional sort through the AriaII cell sorter.
The fusion of UbΔGG to the cytosolic tail of PM marker RFP-TMD23 represents just such an additional sorting signal, while it is the only existing sorting signal in the context of the post-translationally inserted PM reporter Box-GFP.
It would also be possible to further enhance the efficiency of the screening process by adding an additional sorting step to eliminate cells which exhibited leaky gene regulation under conditions of Dox-mediated repression.
To investigate their antigen-presentation capacity, an additional sorting was performed yielding four different populations of DCs: CD11c+GFP− cells that had not taken up neutrophils during their overnight culture with either eGFPhiRFP−-uninfected or eGFPhiRFP+-infected neutrophils, and CD11c+GFP+-positive cells that had taken up either uninfected or infected neutrophils.
The apparent inability of caveolin-2 to traffic from the Golgi independently of caveolin-1 in uninfected cells [ 25, 26] implies that in a manner yet unknown, the chlamydial inclusions might produce a factor that activates an additional sorting and transport process for caveolin-2.
Since the evolutionarily conserved basic cluster of amino acid residues in the N-terminal segment of ScPex3 is not essential for its transport but has been reported to be important for transport of Pex3 orthologues, we tested whether an additional sorting signal is present in this segment of ScPex3.
You can access additional sort options by right-clicking on any cell in a column and selecting "Sort" from the popup menu.
Mr. Liss said an industry recycling group, The Carton Council, had been created to address the problem by developing additional sorting equipment.
Compare that with Massachusetts, which has a median minimum balance requirement of $6,000 — the highest among the states — but fewer additional sorts of fees (11).
