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Subsequent queries with eubacterial GSII sequences did not retrieve any additional sequences.
N N, an expression clone without any additional sequences, was the control clone.
Comparison of their genomes did not identify any additional sequences that may contribute to host adaptation.
Digestion with Ase I demonstrated that this was not due to any additional sequences in the immediate upstream region.
Second, other than Xa21 sequence, DXT did not carry any additional sequences such as that for marker genes and reporter genes.
Additional searches using these two proteins (using TBLASTN), or the corresponding nucleotide sequences (using BLASTN) against the full genome assembly and available ESTs failed to identify any additional sequences corresponding to RNase T2 homologs.
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Aside from their C-terminal sequences, these proteins do not appear to contain any additional sequence motif for membrane targeting as the C-termini alone from three of these proteins can render eGFP entirely membrane-associated (Figure 4).
We generated tCWCH2 consensus sequences for each gene class as well as for all classes to identify any additional sequence features.
Since this enzyme cuts outside of its recognition site, it removes the recognition site during cloning and thus avoids the insertion of any additional sequence.
Without any additional sequencing, we were able to analyze insertions for the presence of target site duplications (TSDs), an L1-endonuclease recognition motif (Jurka, 1997), and a polyA tail.
In principal, these parameters (Additional file 8) could now be applied to any sample run using our custom array (or the widely-used Agilent 44K Whole Human Genome Microarray) to improve absolute expression quantification for around 80% of genes without needing to perform any additional sequencing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com