After pelleting agarose by brief centrifugation, 2 µg of anti-Sp1 antiserum (test group) or 2 µg of β-actin antibody (irrelevant antibody control) was added to the supernatant fraction and incubated overnight at 4°C with rotation.
Previous studies have demonstrated that SARS-CoV N protein can cross-react with polyclonal antiserum induced by group 1 animal CoVs (8 ).
Two of the virus isolates that were IFA-positive with Congo group antiserum were RT-PCR negative when primers specific for DUGV, CCHFV, Hazara virus, or BHAV, all of which were represented in the antiserum, were used.
The bacteria agglutinated in Shigella group D antiserum but failed to agglutinate in Shigella groups A, B, and C antisera (Becton Dickinson Diagnostic Systems Italia, Milan, Italy).
IHC assays that used a polyclonal anti– N. meningitidis group Y antiserum that is broadly reactive with serogroups A, B, C, W, and Y, and a specific monoclonal anti– N. meningitidis serogroup C antibody, revealed immunostaining in the leptomeninges, lung, and skin tissues (3, 4 ).
Colonies were serologically confirmed by slide agglutination with appropriate group-specific polyvalent antiserum, followed by type-specific monovalent antisera.
Since an antiserum and MAbs to a group-I isolate from Ethiopia (CpCSV-Eth) were available from a previous study [ 2], we purified the virions of CpCSV-Sy, a group-II isolate, and used them for polyclonal antibody (antiserum) and MAb production.
Importantly, this group subsequently reported that antiserum raised against this synthetic peptide could specifically neutralize HIV in vitro [13].
They demonstrated that rabbits immunised with the group A M protein produced antiserum that was cross-reactive with central nerve system antigens in Western blots.
Antiserum or NSS were injected intraperitoneally (i.p). in the respective group of rats twice a week (10 µl/g body weight).
