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According to this mechanism, even a relatively low abundance of antisense transcript can mediate transcriptional repression42.
Marquardt, S. et al. Functional consequences of splicing of the antisense transcript COOLAIR on FLC transcription.
This suggests that sense transcription represses the antisense transcript.
10.7554/eLife.03635.017 Figure 5. HMS2 sense transcription represses the HMS2 antisense transcript and BAT2.
Vigetti, D. et al. Natural antisense transcript for hyaluronan synthase 2 (HAS2-AS1) induces transcription of HAS2 via protein O-GlcNAcylation.
An antisense transcript named FAST-1 (FXN Antisense Transcript – 1), whose sequence overlaps with the CTCF binding site, has also been discovered.
Sandi, M. Identification and quantification of FXN antisense transcript 1 (FAST-1) in friedreich ataxia.
Summarized gene antisense transcript abundances were derived similarly, using the probes from the antisense strand60.
Elevated levels of the FXN antisense transcript (FAST-1) have previously been detected in FRDA.
We reasoned that antisense transcript levels could be used as proxy for the activity of alternative promoters and tested whether the low accessibility of the long genes was associated with low antisense transcript levels.
Long noncoding RNA glypican 3 (GPC3) antisense transcript 1 promotes hepatocellular carcinoma progression via epigenetically activating GPC3.
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