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The antisense strand was labelled by incorporation of [α-32P]-dATP in a single-stranded PCR reaction.
The natural strand was known as the sense strand, and the synthetic one as the "antisense" strand.
One strand, called the sense strand, carries the genetic instructions; it is intertwined with the complementary antisense strand to form the double helix.
If the antisense strand could attach tightly enough and in the right place, it would become a wrench in the gears of the genetic machinery, and stop the virus from replicating.
One of the most difficult was to deliver the antisense strand to the right place at the right moment, after the virus had penetrated the cell, but before it had replicated and escaped to infect other cells.
The sense strand of the targeted intron was highlighted in blue color, and the antisense strand was in gray.
Then GUVs displaying the antisense strand are allowed to tether, spread and rupture to form tethered bilayer patches.
Using the 3' cleaved intron remnant as the template, the antisense strand was synthesized, resulting in the production of 24-nt phased sRNAs.
The lysate was split into four parts: one 100 μL aliquot for input, and three 300 μL aliquots for negative control, sense strand and antisense strand RNA, respectively.
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In contrast, the ECS011 sRNA is encoded on the cis-antisense strand of the IS128 sRNA.
Thus, only the antisense-strand probe set was further analyzed in this study.
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