Sentence examples for antisense fragment from inspiring English sources

Compared to the parental cell line, the new strain (CHO-AsUH2), which was transfected with a 199-bp antisense fragment derived from the mouse CMP-Neu5Ac hydroxylase cDNA, showed an 80% reduction in hydroxylase activity.

In an effort to mimic the metabolic changes effected by this fed-batch strategy at the molecular level, 293 HEK cells were engineered via stable transfection with an antisense fragment of the rat phosphate-dependent glutaminase (PDG) gene.

The method is based on PCR amplification of a target sequence with two sets of primers to generate a sense and an antisense fragment.

The In411 antisense fragment was amplified from pMU3 construct using BsrGI In711 (Fwd 5'-AGCTGTACAAGTAAAGCGGCCGCGACTCTAGATC-3') and MluI In411 (Rev 5'- GTandGCGTAAAAAATTCCTTATAGCCAGGTCTAAAATGGATCCGAAGACCACAAACAAG-3') and was cloned to make pMU2-In411, the antisense fragment into the BsrGI-MluI sites of pMU2 vector.

The sense fragment, a 1-kb GUS spacer gene fragment, and the antisense fragment were ligated into the plant T-DNA binary vector pCGN and driven by the Cauliflower Mosaic Virus 35S promoter.

How the L antisense fragment is generated remains mysterious.

The homologous region of ONG1 and ONG2&3 (0.72 kb), and part of ONG4 (0.7 kb) were fused and used as an inverted repeat structure of sense and antisense fragments of ONGs.

Sense and antisense fragments of AtTOR were amplified by RT-PCR (reverse transcription-polymerase chain reaction) using gene-specific primers (Table 1).

Our experience to date is that if we can successfully amplify the sense and antisense fragments we have always been able to generate the clone of interest.

For cloning, the ss-tailed sense and antisense fragments are mixed with an expression vector harboring the complementary ss-sequences and allowed to anneal.

This is because that antisense transcript is expected to have a different intron/exon structure as its sense transcript, and we did not find significant number of introns in the antisense fragments.