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An aptamer (cyan) is engineered to harbor an antisense element (magenta) in its variable region.
It should be emphasized that the antisense element is composed entirely of DNA.
Given that the loop is not involved directly in target binding, it poses a good site to incorporate an antisense element.
By conjugating such aptamer to the fluorogenic substrate of MgZ, the substrate is sequestered in an extended conformation (such as a duplex) by hybridization with the antisense element.
The activity of three antiviral genes, an shRNA directed at tat/rev, a long RNA antisense element, and a membrane-anchored antiviral C peptide (maC46) were compared.
In the presence of the target, however, the aptamer pulls away the antisense element from the substrate and folds into the target-bound conformation.
Similar(33)
A series of antisense elements were screened for ADP-induced substrate cleavage.
Using a new engineering approach, the aptamers were incorporated with antisense elements and annealed to the substrates to form ASAP complexes.
Given that the cleavage and fluorescence-signaling performance of MgZ were poorer when coupled with the ASAPs than with the unmodified substrate, the sensor performance might be further improved by screening a larger library of antisense elements via in vitro selection.
Antisense elements are in italics.
Homologous snoRNAs in different vertebrate species share the same antisense elements.
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