It is believed to exhibit waterproofing, antioxidant, temperature-regulating, and anti-infective functions 8.
The optimal SWE conditions for simultaneous maximization of TP, TF and antioxidant activity were temperature of 201.5 °C, extraction time of 15.8 min and pure water as extraction solvent, while predicted values of TP, TF, IC50, TEAC and EC50 at this experimental point were 7.98 g GAE/100 g DW, 4.93 g CE/100 g DW, 13.38 μg/mL, 38.34 mM Trolox/100 g DW and 41.66 μg/mL, respectively.
According to these facts, minerals Na, K, Ca, Mg, the most important antioxidant Zn, and temperature (34 °C) of Aab-e-Shifa water are probably very much helpful for the treatment of many diseases especially for skin such as scabies, acne, ringworm, and arthritis.
For fatty acid (FA) analysis, ~100 mg of lyophilized algal biomass was re-suspended in 5 mL chloroform: methanol (2 1 v/v) containing pentadecanoic acid (15:0, 2.0 mg/mL; Sigma) as an internal standard and 0.5 mg/mL butylated hydroxytoluene (BHT) as an antioxidant at room temperature for 24 h.
In particular, there are differences in the muscle type (diaphragm, gastrocnemius, EDL, or soleus muscles), the species (rats, mice, or dogs), the nature and concentration of the antioxidant, and the temperature and incubation periods.
The performed method allows extracting the studied antioxidants at low temperature in a short time without degradation of phosphite antioxidant Irgafos 168.
One group of such methods is based on determination of oxidation stability of oil with an antioxidant at higher temperatures (Schaal oven-storage test [ 14], active oxygen method, Rancimat test [ 15], Oxidograph test [ 16]).
Ferrulic acid is one of powerful antioxidant investigated regardless of temperature and probably may stabilize γ-tocopherol from high temperature destruction.
The objective of this work was to optimise different parameters (solvent concentration, time and temperature) for antioxidant extraction from kinema, demonstrated by total phenol content (TPC) and DPPH-scavenging activity (DSA), using response surface methodology.
The experimental values were close with those predicted values, indicating suitability of the model employing RSM for optimizing the extraction time and temperature on antioxidant activity from P. porrigens.
The effect of drying temperature on antioxidant activity of mango seed kernels is shown in Figure 4a.
