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Sequential therapy, a new regimen administering antimicrobials in a given sequence rather than all simultaneously, has generated worldwide interest.
Use of multiple antimicrobials in a single record was not uncommon; 141 (20.6%) records indicated that treatment included ≥ 2 antimicrobials (Table 2).
Such commensal bacteria can be reservoirs for transferable resistance genes and thereby reflect the selective pressure from use of antimicrobials in a population [ 9, 10].
Thus, a prudent use of antimicrobials in a combination with good hygienic conditions is required and further studies are necessary to investigate the role of intestinal E. coli as a possible reservoir of antimicrobial resistance.
An IV to PO conversion quality measure has also been proposed for highly bioavailable antimicrobials in a clinically stable population; however we are unaware of recommendations that incorporate both concepts into a single measure [ 53].
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For all of the three media, MIC was defined as the lowest concentration of an antimicrobial in a plate, which inhibited growth of the test strain.
Both HeLa cells and HepG2 cells were cultured in high glucose DMEM media with 10% FBS and 1% antibiotic and antimicrobial in a humidified atmosphere containing 5% CO2 at 37 °C, according to the established protocol (ATCC, Rockville, MD).
Previously, we investigated leaf extracts of 120 different Rhododendron species for their efficacy as antimicrobials in killing a variety of Gram-positive and Gram-negative bacteria [ 25].
(Although "antibiotics" and "antimicrobials" are sometimes used interchangeably, antibiotics are actually a subset of antimicrobials). The new legislation, which is expected to become law by 2022, bans the use of antibiotics for animals that are important for human medicine and prohibits the use of any antimicrobials in livestock without a prescription from a vet, reports Feedstuffs.
The Centers for Disease Control recognizes that "widespread use of antimicrobials in agriculture has resulted in an increase in resistant infections in humans".
However, there is an increasingly robust consensus that unnecessary use of antimicrobials in animals and agriculture is a significant concern, and that minimising the unnecessary and inappropriate use of antimicrobials is an essential component of global AMR strategies.
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