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Clinical, microbiological and antimicrobial data were collected.
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The methodology used to restructure antimicrobial data was validated by comparing the sensitivity profiles prepared by military treatment facilities to the NMCPHC sensitivity profiles from the restructured data.
Antimicrobial data was acquired from the pharmacy department as total grams dispensed to the unit per month (see Additional file 1).
Antimicrobial data is available through national systems; the Prescribing Information System (PIS) provides data for primary care prescribing at GP Practice level while the Hospital Medicines Utilisation Database (HMUD) provides information on antimicrobial use at hospital level only.
Antimicrobial drug use in outpatient settings was ascertained by the most comprehensive network for European surveillance of antimicrobial consumption (ESAC), and antimicrobial resistance data were obtained from the European surveillance system EARSS).
For all skin infections on which antimicrobial drug data were available, 264 of 382 (69.1%) patients were prescribed antimicrobial drugs.
Antimicrobial exposure data were summarized as ADDs administered parenterally or in feed between arrival and second sampling, by class of antimicrobial drug, and by route of administration (beta lactams, macrolides, phenicols, quinolones, tetracyclines, and sulfonamides for parenteral exposures and tetracyclines for in-feed exposures).
Patients with acute-onset postoperative endophthalmitis caused by Gram-negative bacteria occurring between January 2005 and December 2010 and antimicrobial sensitivity data were obtained from the microbiology database.
Antimicrobial consumption data were obtained from the Spanish National Health System.
Total antimicrobial use data were analysed according to the WHO ATC/DDD methodology and expressed in DDD/1000inhabitants/day (DID).
The proposed guidelines for analyzing and presenting cumulative antimicrobial susceptibility data were published by the Clinical and Laboratory Standards Institute (formerly NCCLS) in 2002.
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