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Assays were performed using twofold serial dilutions in antimicrobial concentration.
The intrinsic rate of disinfection was assumed to follow first-order dependence on antimicrobial concentration.
For each antimicrobial concentration, at least four replicate wells were considered.
The reported MBC was the lowest antimicrobial concentration that resulted no colony formation on the agar.
The MIC was determined by identifying the lowest antimicrobial concentration that completely inhibited growth in all replicate wells.
A relationship between antimicrobial concentration and microbial population reduction was not found and from this point of view; the use of lower antimicrobial concentrations can be recommended.
Similar(34)
In the absence of adequate antimicrobial concentrations, irreversible tissue destruction ensues [3].
The third set (antimicrobial concentrations) helped establish the optimum concentration of trans-cinnamaldehyde in the coating.
The reported MBCs were the minimum antimicrobial concentrations for which no colony formation was observed by visual inspection.
All antimicrobial concentrations were plotted as molarities to facilitate comparisons between compounds (molecular weights are listed in the "Materials and methods" section).
Indeed, at these antimicrobial concentrations, at least 94% of CHAT was rapidly solubilised (within 15 min), again as judged by ultrafiltration and ICP-OES analysis (Fig. 3a).
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